How quickly does Autopilot take effect?

Autopilot begins analyzing data as soon as you activate it for each campaign.

We take action on a nightly basis (Pacific time) based on your updated ad performance data for that day. Autopilot typically starts suggesting changes after 3 to 5 days for fast moving SKUs.  Slower products will not have as much data to analyze and can take as long as two weeks before suggestions populate.

The Autopilot will use historical data, but if your account is brand new, PPC.tools needs to get bid history data before it can make meaningful decisions.